| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.063114 |
| Chromosome: | chromosome 3 |
| Location: | 2085334 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156250 | FAP186,POC13 | Proteome of centriole protein 13; (1 of 5) PF04366 - Las17-binding protein actin regulator (Ysc84) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTTACGGAACTGGCGATAGGTGTAATGGGAGGATGGTGCCTGCAGCT |
| Internal bar code: | GATAACTACTTGGTCTTTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2255 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGAACTGGTCTGAGTGA |
| Suggested primer 2: | AGAATCGAGATGAGCAGCGG |