| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063141 |
| Chromosome: | chromosome 16 |
| Location: | 3704991 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680232 | (1 of 1) PTHR12509:SF8 - SPERMATOGENESIS-ASSOCIATED PROTEIN 4 | 3'UTR | |
| Cre16.g680588 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCCTGATCGGCTGGTTAGGGCTGCGTTCTTTTGCTGACAAGCGTTCA |
| Internal bar code: | TTTTGCGTTTGAAATATTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3439 |
| LEAP-Seq percent confirming: | 97.4026 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTTCCGTTGCCGTTCAG |
| Suggested primer 2: | AGCAGCAGGTGGACAAGATC |