Insertion junction: CLIP2.063146_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.063146
Chromosome:chromosome 12
Location:7125846
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g801463 (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCCCAGCTGTCCCTCCCAGCTGTCCCTTCCAGTCGTCCCTTCCAGTT
Internal bar code:TATAACTTGAATCCTCTAGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:4695
LEAP-Seq percent confirming:89.2157
LEAP-Seq n confirming:91
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:102

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGTGACAAACAGGGGCAA
Suggested primer 2:AGGTCGGTGCTAAGTTTGGG