| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063147 |
| Chromosome: | chromosome 7 |
| Location: | 4134828 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342550 | SMM28 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR10108:SF827 - METHYLTRANSFERASE-LIKE PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGGCGACCTGATTTGTACAAGTTCAAGGAGACGTGACCGCATCATGC |
| Internal bar code: | TATGGAAAGATACAGCGGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1008 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCACCGAGAATGGGCTG |
| Suggested primer 2: | CACACACACACACACGCAAT |