| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063149 |
| Chromosome: | chromosome 3 |
| Location: | 4594850 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176550 | PRMT5,PRM5 | (1 of 1) K02516 - protein arginine N-methyltransferase 5 (PRMT5, HSL7); Protein-/Histone-arginine N-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACTCACACCGGCTGGCGCGCAAGTGAACAGAGTCGCGAGTTTCTCTTT |
| Internal bar code: | TCCGAAGGCAGCGCGAGTGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGTGGGAGAACGGAATG |
| Suggested primer 2: | AAGGACGTGTTGCTGTCGAT |