Insertion junction: CLIP2.063175_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.063175
Chromosome:chromosome 12
Location:8260684
Confidence (%):40
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACTGCAACGCTGCGGTCCGTCCCCAACATCACCAATGCGAGAAATCTG
Internal bar code:CGCATCGTAGGGATGTATTTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1807
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTTTGCCAGTTGCTGACG
Suggested primer 2:TGTGATTGTGCACCGCTTTG