Insertion junction: CLIP2.063180_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.063180
Chromosome:chromosome 5
Location:822831
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g245950 DRP1 Dynamin-related GTPase; (1 of 2) PF01031//PF01926//PF02212 - Dynamin central region (Dynamin_M) // 50S ribosome-binding GTPase (MMR_HSR1) // Dynamin GTPase effector domain (GED) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTGCAACTCCCGAGCACTTCCATCGCGACCCCTCACCTTCTGTGCGAT
Internal bar code:TTGTAGATTCTTCTTTTACGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2101
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGCGTCGGAGTACTAACC
Suggested primer 2:CTCGTCAAAGAAACCCCCGA