| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063211 |
| Chromosome: | chromosome 4 |
| Location: | 2394152 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221301 | CYCD5 | D-type cyclin; (1 of 10) PTHR10177 - CYCLINE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATAGCACGGATGTAGTTTCTGTTCATGACAGATCAGTTAATGCCAAAC |
| Internal bar code: | TTGTCCATTAGTAAAGGGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2955 |
| LEAP-Seq percent confirming: | 98.8764 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAACCATGATGCCTGAT |
| Suggested primer 2: | GCAGCTAGAGTAGGAAGGCG |