| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063233 |
| Chromosome: | chromosome 9 |
| Location: | 6747629 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g417200 | (1 of 1) K15030 - translation initiation factor 3 subunit M (EIF3M) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTGTTGGCCCGGGTTTGGGGCGGGTTTTGTCCGAATTCCAATGGAGA |
| Internal bar code: | GCAAAAAGCCTTATGAATTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5846 |
| LEAP-Seq percent confirming: | 98.8636 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAGAGGGGCGAGTAAGCA |
| Suggested primer 2: | TCCGCATATTCGTACCACCG |