| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.063233 |
| Chromosome: | chromosome 9 |
| Location: | 6747631 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g417200 | (1 of 1) K15030 - translation initiation factor 3 subunit M (EIF3M) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCGCCCCAGACATTAGCACCAAGGAGGAATGTCTAGGAAACGGCCAT |
| Internal bar code: | GTACGCCAGCGTTACGAATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3482 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCATATTCGTACCACCG |
| Suggested primer 2: | ACTAGAGGGGCGAGTAAGCA |