| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.063241 |
| Chromosome: | chromosome 6 |
| Location: | 4682823 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278700 | Conserved Ankyrin-Repeat Protein; (1 of 1) PTHR24193:SF69 - IQ MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN LOC642574 HOMOLOG-RELATED | 3'UTR | |
| Cre06.g278750 | UCP1 | (1 of 1) PTHR24089//PTHR24089:SF200 - FAMILY NOT NAMED // MITOCHONDRIAL UNCOUPLING PROTEIN 1; mitochondrial uncoupling protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGCTGCTGCCCGTTTCGCGCATCGCACCCGTCCACCCAGTCCATGAT |
| Internal bar code: | ATTGTAGTAGCTCTGTCACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2148 |
| LEAP-Seq percent confirming: | 73.0769 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAAAAGGGCAGTTCCTG |
| Suggested primer 2: | AGGTACAGTGCAACGTGAGG |