| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063245 |
| Chromosome: | chromosome 14 |
| Location: | 3534985 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630787 | 5'UTR | ||
| lncRNA_TCONS_00106449 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCAACTTGAACCCATGGCAATGCAGCCCGCGGTCGCGGCTTCTTCTG |
| Internal bar code: | CGAGAGTACCTTGCTCATGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2502 |
| LEAP-Seq percent confirming: | 74.0741 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAACAGCAAGCAGGGTC |
| Suggested primer 2: | CTGGCGCTGCTCTTTTTGTT |