| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063274 |
| Chromosome: | chromosome 3 |
| Location: | 7653465 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204150 | FAP167,IFT80 | Intraflagellar transport protein 80; (1 of 1) PTHR24098//PTHR24098:SF0 - FAMILY NOT NAMED // OSEG5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACACATGCTGCACGCTACATTGTAAATCGGTCTGTGGCCTCATGCTG |
| Internal bar code: | AGTTGACCAAGATTTTCATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCCTCACCTCTTCCCTCC |
| Suggested primer 2: | TAGGCTCCAAGCACAGAAGC |