Insertion junction: CLIP2.063278_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.063278
Chromosome:chromosome 13
Location:4800636
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g604700 PGP2 (1 of 1) K08744 - cardiolipin synthase (CRLS); Phosphatidylglycerolphosphate synthase 3'UTR
Cre13.g604750 CGL94 zinc finger family protein; (1 of 37) PF13639 - Ring finger domain (zf-RING_2) outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAGAGTGACGGCGCTGTGCATGATCGGTGATGGGGTGAAGCGCGAGCG
Internal bar code:GTACATTACCGGCTTTCGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1430
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGGCTATGGAAGGGGAT
Suggested primer 2:CCGAGTGACCGAATCGAGAG