| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.063310 |
| Chromosome: | chromosome 12 |
| Location: | 2760458 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504200 | RPS23 | (1 of 1) K02973 - small subunit ribosomal protein S23e (RP-S23e, RPS23); Cytosolic 80S ribosomal protein S23 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGGGCGGGCAGTGCGAGAATGGGGGCGGCTCATGCTACCAGGGCTTG |
| Internal bar code: | GGAGCGCTCAAAACAGCTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2562 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACATTCTGGTCGTCCCA |
| Suggested primer 2: | CGAAGGCGGCAATCTTCTTG |