| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063311 |
| Chromosome: | chromosome 6 |
| Location: | 6460590 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293850 | CAG2 | Gamma carbonic anhydrase 2; (1 of 1) PTHR13061//PTHR13061:SF0 - DYNACTIN SUBUNIT P25 // GAMMA CARBONIC ANHYDRASE-LIKE 1, MITOCHONDRIAL-RELATED | 3'UTR |
| Cre06.g293900 | (1 of 1) PTHR23333:SF4 - UBX DOMAIN-CONTAINING PROTEIN 11 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTACGTCTACCAGGCACGAAACAGCCATCCAGCCCGCTCACACCGGA |
| Internal bar code: | AACGAGGGGTAAGGGAGTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2611 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTACCGTACCCCCAACA |
| Suggested primer 2: | GGGTGATACTAGGGCGTTCG |