Insertion junction: CLIP2.063312_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.063312
Chromosome:chromosome 3
Location:5413290
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g183800 (1 of 1) PF10225 - Uncharacterized conserved protein (DUF2215) (DUF2215) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGTGCAAGCGCTTTCGGTGGAAAGCGCCCCAAACCCAGAAAACTGGA
Internal bar code:CGTGTCGGGATTACCGCTTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4660
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGGATCTCTTCGCCCCTA
Suggested primer 2:CCCACACGACACTGACATCA