| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063313 |
| Chromosome: | chromosome 13 |
| Location: | 1229140 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570300 | UBC24 | (1 of 1) K13960 - ubiquitin-conjugating enzyme E2 T (UBE2T, HSPC150); E2 Ubiquitin conjugating enzyme | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCTTCACAAGCGTAGCGTTCACCGTTCACCGCCATGGCGCTCAGCGC |
| Internal bar code: | CATTTGGGCGTGAGTTTGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2071 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACAAGTGATGTGCTGCA |
| Suggested primer 2: | AGGCGGAGAAGGTATTGTGC |