| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063324 |
| Chromosome: | chromosome 6 |
| Location: | 3917299 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278192 | (1 of 1) PTHR15627:SF8 - DTW DOMAIN-CONTAINING PROTEIN 1 | 3'UTR | |
| Cre06.g278193 | gene_edge/outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACATTTTGTTACAACTTGCAGGCTAACAGGCTTACACTCGGGTGCCG |
| Internal bar code: | AAGCGAGGAGGGAGTGAACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6349 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTATTGCGCTGTGAATG |
| Suggested primer 2: | GGACGAGATTGCATTGCGAC |