| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063333 |
| Chromosome: | chromosome 3 |
| Location: | 4344826 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174600 | (1 of 3) PF13771 - PHD-like zinc-binding domain (zf-HC5HC2H) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTATGTAGGCGTTACCCCCAGCATGGTAAGTCCTGGCAGGGGAAACCCT |
| Internal bar code: | CCATAGCCTTCTGAGAAGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1044 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGAACGTACAGTGCTGT |
| Suggested primer 2: | AGAGGCAGCTGATGGAAACC |