Insertion junction: CLIP2.063341_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.063341
Chromosome:chromosome 16
Location:4746643
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g688050 (1 of 1) PTHR32091//PTHR32091:SF0 - FAMILY NOT NAMED // EUKARYOTIC INITIATION FACTOR 4B-RELATED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGCAAGGGCCGGTTGAAGGAGACTCGGGCACAAAAGGTGCGATCGGC
Internal bar code:CAAGGCATTCTAAACAGAAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2460
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGGTAGCGGACAAAGCAT
Suggested primer 2:GCCTCCTGCAAACACACAAG