| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063354 |
| Chromosome: | chromosome 13 |
| Location: | 3869388 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589550 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCTAGAGCGCTAGCGGGGACAGGCCCAACCAGAGAGCGGGGACAGCG |
| Internal bar code: | TCCTGTAGGAAGTTATTCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2544 |
| LEAP-Seq percent confirming: | 95.3125 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTACAAAAACGCCGTGTT |
| Suggested primer 2: | ACTCCCACACCGTAGATGGA |