| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063365 |
| Chromosome: | chromosome 7 |
| Location: | 2683446 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330900 | (1 of 140) IPR005135 - Endonuclease/exonuclease/phosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACACACACACACACACACACACACACGCACACGCACACGCACACGCA |
| Internal bar code: | GGACGTGCCAGTTTCCTAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5354 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGACGTGGTGTATGTGCT |
| Suggested primer 2: | ATAGAATGCTGCGGACCTCG |