Insertion junction: CLIP2.063387_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.063387
Chromosome:chromosome 16
Location:4909205
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g686800 PTA2 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter, splice variant b 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATAAAGCATCATGCACAAGACCAGGCAGTCATTTCCAAACCCCAAGCTA
Internal bar code:CAGAGGTTTGCCGCTTTAGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2117
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGCAAGAACTACCAGGTG
Suggested primer 2:GAAACACCAACCGTCTTGCC