| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.063397 |
| Chromosome: | chromosome 16 |
| Location: | 2513862 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660601 | (1 of 1) IPR000772//IPR003609 - Ricin B, lectin domain // PAN/Apple domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGATTGAGCTTGTGTACTGGCACTGCTAAAATAATCTCATGAAAAAG |
| Internal bar code: | ACGCTGTTATGACTTTCAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 897 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGTAGCACGTAAAGGTT |
| Suggested primer 2: | TCTCCTCCTCCTTCTCCTGC |