| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063403 |
| Chromosome: | chromosome 10 |
| Location: | 3431983 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444216 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTCACCAGCCGCCGCACACCCGCCTCGTCCAGCTGCGCGGCAGCAGA |
| Internal bar code: | TTGTGTGTATGGTTAGTTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 39.2857 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGAGTACGAGGTACGAG |
| Suggested primer 2: | GTTCATTATGTCGGCGCCAC |