| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063404 |
| Chromosome: | chromosome 7 |
| Location: | 3632028 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337150 | RFC2 | (1 of 1) PTHR11669:SF5 - REPLICATION FACTOR C SUBUNIT 2; DNA replication factor C complex subunit 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTGTGATGCGAGAGTACAGCCCCAAACTGGAGTGTGTGTATATCAAA |
| Internal bar code: | GCCTGAACGTGGCCGTGCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2735 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGCAGCATTAGGTAGCTA |
| Suggested primer 2: | GCAAGCAGGAGTGTGTTTGG |