| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063407 |
| Chromosome: | chromosome 16 |
| Location: | 2259845 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658600 | SPC25, SPCB25 | (1 of 1) K11550 - kinetochore protein Spc25, animal type (SPBC25, SPC25); Putative kinetochore protein Spc25 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCCTTGCGCCAGCGATGCTTCATGGCAAGCCTTGACCGCTGCTTCAA |
| Internal bar code: | ACGTCCACGAAGTTAGTACATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3123 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGGGAGAGCCACCTTTC |
| Suggested primer 2: | CCCTGGTCAAAGTCCTGGAC |