Insertion junction: CLIP2.063409_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.063409
Chromosome:chromosome 8
Location:1891359
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g367600 ASL1,CYSL,CYSM,OASTL1 (1 of 3) 2.5.1.47 - Cysteine synthase / OAS sulfhydrylase; O-acetylserine (Thiol)-lyase/cysteine synthase 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGGCAGACGGGGAATCCTTGCAGCATGTCTGGCTGTGCCAGCTTCAAC
Internal bar code:TTAAGATCAAAGCATCAGCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2665
LEAP-Seq percent confirming:97.0588
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:68

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCTAGCACATTGGAGGTT
Suggested primer 2:GCAATGCCGTGTAGATGCTG