| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.063435 |
| Chromosome: | chromosome 3 |
| Location: | 3201921 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165200 | APG7 | Autophagy-related protein, ubiquitin-activating enzyme E1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCAGCAGCAGCAGCAGCAGCAGCAGCAAGGCTGGCAGAAGGCGGCGC |
| Internal bar code: | TAATGTCAAGTATATGCCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGATGTCTCGGCACAGG |
| Suggested primer 2: | CATCCGGTCCTTCAAGCAGT |