| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.063446 |
| Chromosome: | chromosome 13 |
| Location: | 3096963 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584551 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATCTTGCGGCCGCGCAGGATGCCAGGCGGCACTTCCATGTTGTAGCTG |
| Internal bar code: | TCGCTAAGATCCAATTCATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 36.3636 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGTCTGCTTGATGCTGT |
| Suggested primer 2: | GTCTTCCCACAGCACGTACA |