| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063450 |
| Chromosome: | chromosome 4 |
| Location: | 3986578 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g232006 | gene_edge/mRNA_edge/5'UTR | ||
| lncRNA_TCONS_00242258 | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTATGGATGTTTCTGCGCACATGGCTTACATAAGTGCACAGTAATGA |
| Internal bar code: | CGGGGTCCTGGTTCGAGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1281 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCAGGCAGGTCGTTAACA |
| Suggested primer 2: | ACACCGCCACTCATACACTG |