Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | + |
Strain: | CLIP2.063452 |
Chromosome: | chromosome 11 |
Location: | 3874932 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g479600 | MHX1,CAX6 | (1 of 1) K05849 - solute carrier family 8 (sodium/calcium exchanger) (SLC8A, NCX); putative Ca2+/Na+ exchanger | 5'UTR |
Cre11.g479650 | CHIP,CHIP1 | (1 of 1) K09561 - STIP1 homology and U-box containing protein 1 (STUB1, CHIP); Carboxyl terminus of Hsc70-interacting protein | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACATCCTTCGACGGCTGCGCGGAGACTTCACGCGACGAGTCCTGGTG |
Internal bar code: | CTTTGTTATTTATGGGTCTGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2459 |
LEAP-Seq percent confirming: | 97.2222 |
LEAP-Seq n confirming: | 35 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCAATGACTTGATCGCAGG |
Suggested primer 2: | TGGGCAGCATGTTGAATCCT |