Insertion junction: CLIP2.063452_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.063452
Chromosome:chromosome 11
Location:3874932
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g479600 MHX1,CAX6 (1 of 1) K05849 - solute carrier family 8 (sodium/calcium exchanger) (SLC8A, NCX); putative Ca2+/Na+ exchanger 5'UTR
Cre11.g479650 CHIP,CHIP1 (1 of 1) K09561 - STIP1 homology and U-box containing protein 1 (STUB1, CHIP); Carboxyl terminus of Hsc70-interacting protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACATCCTTCGACGGCTGCGCGGAGACTTCACGCGACGAGTCCTGGTG
Internal bar code:CTTTGTTATTTATGGGTCTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2459
LEAP-Seq percent confirming:97.2222
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAATGACTTGATCGCAGG
Suggested primer 2:TGGGCAGCATGTTGAATCCT