| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063459 |
| Chromosome: | chromosome 6 |
| Location: | 2866731 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272400 | (1 of 1) PTHR11247:SF1 - DOLICHYLDIPHOSPHATASE 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTAGTCATTAGCAATTCAATTTGGAAGTCGGGGCGTTGACGATGCG |
| Internal bar code: | GACTTTCTTCCCGGTTTGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3031 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTTCTGTCTTGCGCAAA |
| Suggested primer 2: | GCGCAATCGACTCAAATCCC |