Insertion junction: CLIP2.063461_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.063461
Chromosome:chromosome 1
Location:1879954
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g800035 (1 of 2) PTHR12436:SF4 - LEUKOCYTE RECEPTOR CLUSTER MEMBER 8 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGGGTGGGGTCGTAGGGGTGGGCGTGCGGATGTGATTCGAAGCACAGG
Internal bar code:CCTCGATCTGTGCTATACCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:698
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCAGAGTCTAGAGGGCTT
Suggested primer 2:ACACACACACACACACCTGT