| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063471 |
| Chromosome: | chromosome 10 |
| Location: | 4755842 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452600 | (1 of 1) PTHR33911:SF1 - RRNA-PROCESSING PROTEIN EFG1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCATCCTCATGCAAGAACCCTCCCGAACGAGACCCCCCTACCTATGCA |
| Internal bar code: | ACGGTACCAGGCAGCAGTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1609 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGAAGGGTAGATACGGC |
| Suggested primer 2: | ACACACAACGCACCTACACA |