| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.063472 |
| Chromosome: | chromosome 3 |
| Location: | 7622850 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204500 | CSE17 | Precursor of chemotactic amidated peptide | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACGGCGCGCACGTGTGTGAGAGCCCTTTGGCATGCTAAATTACTTAT |
| Internal bar code: | GGGCGTTTATAGTTCCAATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1796 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACATGACGCGAAGAACCA |
| Suggested primer 2: | CGTCCTCAACCTGACACCTC |