| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.063477 |
| Chromosome: | chromosome 2 |
| Location: | 4340262 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105150 | (1 of 1) 4.1.1.19 - Arginine decarboxylase / L-arginine carboxy-lyase | 5'UTR | |
| Cre02.g105200 | (1 of 2) K12382 - saposin (PSAP, SGP1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCCCTAGTCATTTGGCCTGGCTCCGCCGAAGCATGCATTAAGAACTC |
| Internal bar code: | GTATCCCTAGTGACTAACGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 90.3226 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGAGAGCAGAAGAGGG |
| Suggested primer 2: | ATTGAGTACTACGCTCGCCG |