Insertion junction: CLIP2.063485_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.063485
Chromosome:chromosome 1
Location:3764991
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g024550 STK (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; Serine/threonine protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGACCCCGGCTGGGCGGTACTGACGTCAAACTGCGTCAAAGAACTGTC
Internal bar code:CCGTTGGGGTCCGTGAAGATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1449
LEAP-Seq percent confirming:95.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCTTGGGAACACACTTTG
Suggested primer 2:TAAGCAAGATGTGGGCGGTT