Insertion junction: CLIP2.063491_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.063491
Chromosome:chromosome 10
Location:847849
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g423450 PPCDC1,COAC1, COAC1,COA4 (1 of 1) 4.1.1.36 - Phosphopantothenoylcysteine decarboxylase / N-((R)-4'-phosphopantothenoyl)-L-cysteine carboxy-lyase; Phosphopantothenoylcysteine decarboxylase, CoA biosynthesis outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCACCTGATGTGGAAGCTTGTTGTTTGCGTCTTCGGTGGTGGTGAAG
Internal bar code:GCGGACAGTCAAAGGTTCTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1951
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGGGTCTCCAACAATGTA
Suggested primer 2:CCGTTGCATTACGCTAGTGC