Insertion junction: CLIP2.063494_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.063494
Chromosome:chromosome 6
Location:214326
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g250450 (1 of 5) K14347 - solute carrier family 10 (sodium/bile acid cotransporter), member 7 (SLC10A7, P7); Sodium:bile acid symporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGGAATAAAAAGGGGCTCCTCTGGTCCAAAGGCACGTGGCAATGGTCT
Internal bar code:TTGTTTTTGAGCGCGGTTAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2751
LEAP-Seq percent confirming:81.4815
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGGCTCCATGTCTCCTGC
Suggested primer 2:CTTCTTCCTGCTCATGGCCA