| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.063502 |
| Chromosome: | chromosome 17 |
| Location: | 2103642 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802047 | (1 of 14) IPR002156//IPR012337 - Ribonuclease H domain // Ribonuclease H-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAAAAGACCACCGCGCAAACTAGGTACCAAGTCTACTGAACCAGCCA |
| Internal bar code: | GCTATAAACAGAATTCTTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 765 |
| LEAP-Seq percent confirming: | 68.1818 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCTTCCCATTCCACCAGG |
| Suggested primer 2: | ACCTGCCGGTATCTGGTTTG |