| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.063504 |
| Chromosome: | chromosome 1 |
| Location: | 991473 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005750 | (1 of 11) IPR002350 - Kazal domain | 5'UTR | |
| Cre01.g005813 | POB31 | Proteome of basal body 31 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGCTGCGGAGTGCGGGTTTGGTTACAGAGTTATCCTGGCGGGCGTAA |
| Internal bar code: | CATGGTTTCATTATGCAGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5071 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTCGCCAAAGCAACAAA |
| Suggested primer 2: | CTTGTGTGTGGCATGTGTGG |