| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063510 |
| Chromosome: | chromosome 12 |
| Location: | 8689467 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547750 | SCS1,SPCS,SPCS1 | (1 of 1) 2.9.1.2 - O-phospho-L-seryl-tRNA(Sec):L-selenocysteinyl-tRNA synthase / SepSecS; Selenocysteine synthase | 3'UTR |
| Cre12.g547800 | SNE15 | (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; NAD dependent epimerase/dehydratase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTTGCTATAGCCACGGATGCGTGTACGACCGAAAATCGAAAGTGCGCG |
| Internal bar code: | AGCATAATCCGTAAGTGGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2790 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTATGTCACACGTTGGCA |
| Suggested primer 2: | GACACTTGGACTCGTGCTCA |