| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.063520 |
| Chromosome: | chromosome 16 |
| Location: | 3713750 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680944 | (1 of 1) K14539 - large subunit GTPase 1 (LSG1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTGCTACCAAATGGCACAATAGCTATTTTTCAGATAATAACTTCTATC |
| Internal bar code: | TATTGTTTCACGTAAGGTGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTGTGCTTGAAGTTGG |
| Suggested primer 2: | CTCGATAGTGCCGCTGGATT |