| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063526 |
| Chromosome: | chromosome 2 |
| Location: | 7855753 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146050 | ATO2 | (1 of 1) 2.3.1.9 - Acetyl-CoA C-acetyltransferase / Acetoacetyl-CoA thiolase; Acetyl-CoA acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGCGTGCCAATGTTGGCTGGTGCTGCAGAGGAGATGGGGGAGGGCGC |
| Internal bar code: | GCGGAGATGCAACAGAGGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 809 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGAGGGAGGATGCTTCG |
| Suggested primer 2: | CACTGCGGATTCGGTGTTTG |