Insertion junction: CLIP2.063527_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.063527
Chromosome:chromosome 6
Location:1893121
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g264150 MAW11 Membrane-associated hydroxyproline-rich glycoprotein 11; (1 of 1) 2.1.1.53 - Putrescine N-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTTCTAGCGGCCTAGGAGACAGCAAGCACAAACCAGTGCATGGGTGTT
Internal bar code:GTTTACACAAGAGATGGACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3398
LEAP-Seq percent confirming:98.9362
LEAP-Seq n confirming:93
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:94

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAGTGACCTTTCGTCCGG
Suggested primer 2:GAGGGATGTTGGTGTGCTCA