| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.063541 |
| Chromosome: | chromosome 1 |
| Location: | 5216773 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036400 | SDR3 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.313 - Sulfoacetaldehyde reductase | 3'UTR |
| Cre01.g036451 | SMM2 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTATGAGCGGTGACCAGGGATGGTATGGTTGGAACGGACTACGGAGG |
| Internal bar code: | ATTCATTAGTAGTCAGTGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5153 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGCTCTACGATGCGTTG |
| Suggested primer 2: | GCGCGTAAAAGAGCAGTAGC |