| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063552 |
| Chromosome: | chromosome 2 |
| Location: | 3925700 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101950 | TRM2B,TMU2 | tRNA (uracil-5)-methyltransferase; (1 of 1) K15332 - tRNA (uracil-5-)-methyltransferase [EC:2.1.1.-] (TRMT2A) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTAGCGACTGGCACGCAATAACCCGACTATAATTGCACGCGCAGTGTC |
| Internal bar code: | TGCGCCTGCAATGAAAATAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1221 |
| LEAP-Seq percent confirming: | 79.3103 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAACACCGCACCCAATCGA |
| Suggested primer 2: | AGGAGTTAGGAGGATGGCGT |