| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063566 |
| Chromosome: | chromosome 2 |
| Location: | 7141513 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390319 | (1 of 1) PTHR10663:SF42 - PROTEIN BOI2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGGAGCAGCTGCACAGGTGTGTCTATTGGCAAGGCGCTGGTTGAACG |
| Internal bar code: | ATGGTGCAAGTTTCATTTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2341 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCAGGTGCAGCTCATCCA |
| Suggested primer 2: | GCAAGTATGGTCGGGGAACA |